Journal: American Journal of Cancer Research

Article Title: Expression of migfilin is increased in esophageal cancer and represses the Akt-?-catenin activation

doi:

Figure Lengend Snippet: The mRNA levels of migfilin in esophageal cancer samples. Scatter plot displaying migfilin mRNA levels between ESCCs and matched adjacent normal esophageal mucosa in published microarray data sets (GSE23400).

Article Snippet: Tissue microarray comprised of 105 patients with ESCCs was obtained at the First Affiliated Hospital of Anhui Medical University (Anhui province, China).

Techniques: Microarray

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Evaluation of pharmacodynamic responses to cancer therapeutic agents using DNA damage markers

doi: 10.1158/1078-0432.CCR-18-2523

Figure Lengend Snippet: (A) Baseline expression of selected DDR markers was demonstrated and quantified in a colorectal tissue array with 32 tumors (2 cores per case are included) and 15 normal colon tissues. At least 500 individual tumor nuclei were quantified in 95% of the tissue microarray cores. (B-C) Baseline marker quantitation in advanced stage colorectal cancers from patients enrolled in Phase 1 clinical trials at NCI. Median expression and inter-quantile range is indicated for each marker. A minimum of 4,000 individual tumor nuclei were quantitated across at least two nonadjacent slides per biopsy specimen. (D) Baseline expression in human colon adenocarcinoma patient-derived xenografts. At least 5,000 nuclei quantified per model. (E) Baseline expression of selected DDR markers was demonstrated and quantified in 8 colorectal cancer cell lines. Over 1000 individual nuclei were quantified for each cell line. (F) Inter-lesion baseline Rad51 quantitation from patients with advanced stage cancers with two biopsies each collected from the same lesion. *p< 0.05. The dashed line represents our empirically determined baseline value cutoff of 5% of cells ≥ 5 Rad51 foci per nucleus.

Article Snippet: Baseline biological variability was established for each biomarker across 64 individual cores from 32 colorectal (CRC) tumor resections contained in a paraffin-embedded colorectal tissue microarray (TMA; Indivumed, Hamburg, Germany).

Techniques: Expressing, Microarray, Marker, Quantitation Assay, Derivative Assay

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Evaluation of pharmacodynamic responses to cancer therapeutic agents using DNA damage markers

doi: 10.1158/1078-0432.CCR-18-2523

Figure Lengend Snippet: Representative H&E and immunofluorescence (IFA) images from 3 patients with advanced colorectal cancer enrolled in NCI trial NCT01851369 before and 5 days after start of treatment with a DNA damaging therapeutic regimen consisting of TCR102 plus temozolomide administered orally once daily. Red scale bar represents 10 μm.

Article Snippet: Baseline biological variability was established for each biomarker across 64 individual cores from 32 colorectal (CRC) tumor resections contained in a paraffin-embedded colorectal tissue microarray (TMA; Indivumed, Hamburg, Germany).

Techniques: Immunofluorescence

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Expression of PRDM 14 in pancreatitis. (A) Representative IHC images for PRDM 14 in acute and chronic pancreatitis in TMA slide. Scale bars in image, 50 μm. (B) Staining scores and P values for PRDM 14 in tissues, 13 normal pancreas, 115 PDAC , 36 cancer adjacent, 30 Pan IN , six acute pancreatitis, and 58 chronic pancreatitis. Error bars in graphs represent mean ± SD . Student's t ‐test: ** P < 0.01, ns, not significant.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Expressing, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreatitis mouse models. Acute and chronic pancreatitis was induced by intraperitoneal cerulein injection. Saline was used as control. (A) Representative images of the pancreases stained with H&E or Sirius red, and IHC for PRDM 14 are shown. Scale bars in image, 200 μm. (B) High‐power magnification of IHC for PRDM 14 of pancreas in chronic pancreatitis mouse models. The left panel is the enlarged image of the black box in the lower right panel in A. Scale bars in image, 50 μm.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Injection, Saline, Control, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreases with spleen of pancreatitis mouse models. Image of all pancreases with spleen of acute pancreatitis and chronic pancreatitis mice ( n = 6 per group).

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: